Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
dehydroepiandrosterone + NAD+ | Pseudomonas sp. | - |
androst-5-en-3,17-dione + NADH + H+ | - |
? | |
dehydroepiandrosterone + NAD+ | Pseudomonas sp. TB | - |
androst-5-en-3,17-dione + NADH + H+ | - |
? | |
pregnenolone + NAD(P)+ | Pseudomonas sp. | - |
pregn-5-en-3,20-dione + NAD(P)H + H+ | - |
? | |
pregnenolone + NAD(P)+ | Pseudomonas sp. TB | - |
pregn-5-en-3,20-dione + NAD(P)H + H+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas sp. | - |
- |
- |
Pseudomonas sp. | - |
TB | - |
Pseudomonas sp. TB | - |
TB | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas sp. |
native enzyme to homogeneity by streptomycin and ammonium sulfate precipitation, ion exchange chromatography, gel filtration, and affinity chromatography on an NAD+-linked resin, thereby separation from 3alpha-hydroxysteroid dehydrogenase, EC 1.1.1.50 | Pseudomonas sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
88.9 | - |
- |
Pseudomonas sp. |
88.9 | - |
purified enzyme | Pseudomonas sp. |
Storage Stability | Organism |
---|---|
-20°C, 2 mM potassium phosphate buffer, pH 7.0, 50% glycerol, 18 months, 50-70% loss of activity | Pseudomonas sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+ | - |
Pseudomonas sp. | 17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H | - |
? | |
3beta,17alpha-dihydroxy-5-pregnen-20-one + NAD(P)+ | - |
Pseudomonas sp. TB | 17alpha-hydroxypregn-5-ene-3,20-dione + NAD(P)H | - |
? | |
dehydroepiandrosterone + NAD+ | - |
Pseudomonas sp. | androst-5-en-3,17-dione + NADH + H+ | - |
? | |
dehydroepiandrosterone + NAD+ | - |
Pseudomonas sp. TB | androst-5-en-3,17-dione + NADH + H+ | - |
? | |
dehydroepiandrosterone + NAD+ | - |
Pseudomonas sp. | ? | - |
r | |
dehydroepiandrosterone + NAD+ | - |
Pseudomonas sp. TB | ? | - |
r | |
pregnenolone + NAD(P)+ | - |
Pseudomonas sp. | pregn-5-en-3,20-dione + NAD(P)H + H+ | - |
? | |
pregnenolone + NAD(P)+ | - |
Pseudomonas sp. TB | pregn-5-en-3,20-dione + NAD(P)H + H+ | - |
? |
Synonyms | Comment | Organism |
---|---|---|
3beta-HSD | - |
Pseudomonas sp. |
3beta-hydroxysteroid dehydrogenase/isomerase | - |
Pseudomonas sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Pseudomonas sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
assay at | Pseudomonas sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
NAD+ | - |
Pseudomonas sp. |